#Vchat molecular weight sigma software#

Statistical analyses are described in ‘Method details,’ and the data are presented as the mean ± SD. ( H) The time to death (absence of respiration) of the same mice was plotted as a function of time in minutes. ( G) WT and TKO male mice (n = 10) were intraperitoneally injected with a lethal tubocurare dose (0.6 mg/kg body weight), and the number of mice undergoing complete paralysis was plotted as a function of time in seconds.

#Vchat molecular weight sigma software#

( F) WT and TKO male mice (n = 10) were intraperitoneally injected with different tubocurare doses, and the LD 50 of curare was calculated using an online software LD 50 Calculator (AAT Bioquest, Inc, Sunnyvale, CA). ( E) WT and TKO female mice (n = 6) were intraperitoneally injected with tubocurare (0.15 mg/kg body weight), and then core body temperature was measured every 15 min at room temperature. ( D) WT and TKO male mice (n = 6) were intraperitoneally injected with tubocurare (0.4 mg/kg body weight) at room temperature, and then 10 min later shifted to 4☌ for 30 min, with core body temperature measured every 10 min. ( C) WT and TKO male mice (n = 6) were intraperitoneally injected with tubocurare (0.4 mg/kg body weight), and then core body temperature was measured every 10 minutes at room temperature. ( B) WT and TKO male mice (n = 6) were intraperitoneally injected with CPA (10 mg/kg body weight) at room temperature, and then shifted to 4☌ for 30 min, with core body temperature measured every 15 min. ( A) Wild type (WT) and whole-body Tigar knockout (TKO) male mice (n = 6) were intraperitoneally injected with CPA (10 mg/kg body weight), and then core body temperature was measured every 15 min at room temperature. Statistical analyses are described in ‘Method details’ and the data are presented as the mean ± SD. ( H) Indirect calorimetry determination of energy expenditure in the light and dark cycles of the WT and TKO mice. ( G) Indirect calorimetry determination of spontaneous locomotor activity in the AMB + Z dimensions from the WT and TKO mice in the light and dark cycles. ( F) Quantification of daily food intake/body weight in the light and dark cycles of WT and TKO male mice. ( E) Representative images of hematoxylin and eosin staining of soleus and extensor digitorium longus (EDL) skeletal muscle from the WT and TKO mice. ( D) The ratio of lean mass/body weight and fat mass/body weight was determined by EchoMRI at 5 months of age for male WT and TKO mice. Please note that the Ct values for brown adipose tissue were approximately 18, whereas the Ct values for the inguinal white adipose tissue were in the range of 33. ( C) Relative Ucp1 mRNA levels in inguinal adipose tissue from the WT and TKO male mice (n = 4–10) at ambient temperature or after 2 hr 4☌ exposure. ( B) Representative TIGAR, UCP1, and actin protein immunoblot of interscapular brown adipose tissue from two independent WT and TKO male mice maintained at ambient temperature or after 1 hr 4☌ exposure. ( A) Relative Ucp1 mRNA levels in interscapular brown adipose tissue from wild type (WT) and TKO male (n = 6) mice maintained at ambient temperature and after 1 hr 4☌ exposure.

Statistical analyses are described in ‘Methods details,’ and the data are presented as the mean ± SD. ( H) Myl1 Cre and Myl1 Cr>/Tigar fl/fl male mice (n = 6) core body temperatures were measured every 15 min starting at ambient laboratory temperature (0 min) and following placement at 4☌.

( G) Representative TIGAR immunoblot of heart, gastrocnemius muscle (Gastroc), extensor digitorum digitorum longus (EDL), and soleus muscle from the control ( Myl1 Cre) and skeletal muscle-specific TKO ( Myl1 Cre/Tigar fl/fl) mice. ( F) WT (n = 6), TKO (n = 6), UKO (n = 7), and UTKO (n = 8) mice core body temperatures were measured every 15 min starting at ambient laboratory temperature (0 min) and following placement at 4☌. ( E) Representative UCP1 and TIGAR immunoblots of brown adipose tissue from WT, TKO, Ucp1 knockout (UKO), and Ucp1 and Tigar double knockout (UTKO) mice from two independent genotypes each. ( D) Tigar fl/fl and Tigar fl/fl/Adipoq Cre male mice (n = 6) core body temperatures were measured every 15 min starting at ambient laboratory temperature (0 min) and following placement at 4☌. ( C) Representative TIGAR immunoblot of gastrocnemius muscle (Gastroc), epididymal white adipose tissue (EWAT), subcutaneous inguinal white adipose tissue (iWAT), and interscapular brown adipose tissue (iBAT) from the control Tigar fl/fl and adipocyte-specific knockout Tigar fl/fl/ Adipoq Cre mice. ( A) Male mice (wild type n = 10, whole-body Tigar knockout n = 6) and ( B) female mice (WT n = 6, TKO n = 7) core body temperatures were measured every 15 min starting at ambient laboratory temperature (0 min) and following placement at 4☌.